A separation-free electrochemical heterogeneous immunoassay based on the Nafion membrane modified electrode has been developed for the determination of IgM. The anti-IgM antiserum and the o-amino phenol were immobilized on the electrode surface forming a biological sensitive membrane. 发展了一种基于Nafion膜修饰电极构制的无分离步骤的异相免疫分析法,羊抗人IgM抗血清和邻氨基酚通过Nafion膜固定在电极表面,形成生物敏感膜。